Application
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
1HAEo- human airway epithelial cell line is a useful model for the study of epithelial ion transport, secretion and biochemistry.
Cell Line Description
Epithelial Cells
Disclaimer
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
1HAEo- is a human airway epithelial cell line that was immortalized with the origin-of-replication defective SV40 plasmid (pSVori-). The cell line retains characteristic features of normal differentiated airway epithelial cells including a cobblestone morphology, cytokeratin expression, the ability to form tight junctions and directional ion transport. It is unknown whether the donor had cystic fibrosis or not, however the cells do not contain the δF508 mutation in the CFTR gene or other CFTR mutations presenting 98% of all CF mutations (1). CFTR sequence analysis revealed only wild-type CFTR sequences, supporting the conclusion that 1HAEo- are derived from normal (i.e. non-cystic fibrosis) tissue (1).
Quality
Each vial contains ≥ 1X106 viable cells.
Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells are negative for mycoplasma contamination.
Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Storage and Stability
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
