AC16 Human Cardiomyocyte Cell Line

AC16 Human Cardiomyocyte Cell Line

Stock Code: 3572316
Manufacturer Part No: SCC109
Order Now for 21 day delivery
Click Add to Quote for price and delivery
Quantity: - +

Application


Research CategoryCardiacCardiovascular DiseaseToxicity


This product is available for sale to academic institutions or not-for-profit entities for research use. For information on commercial licensing please contact licensing@emdmillipore.com.


Cell Line Description


Cardiomyocyte Cells


General description


AC16 Human Cardiomyocyte Cell Line is a proliferating cell line that was derived from the fusion of primary cells from adult human ventricular heart tissues with SV40 transformed, uridine auxotroph human fibroblasts, devoid of mitochondrial DNA . After selection in uridine-free medium to remove unfused fibroblasts, the resulting fused cells were further subcloned and subsequently screened for the presence of SV40 large T-ag, ?-myosin heavy chain (?MHC) and connexin-43 (CX-43) .AC16 cells are proliferating and differentiation of the cells may be controlled by altering culture conditions and by silencing the expression of SV40 T-Ag . The cells can be used to study cardiac gene expression and function, during normal development and in pathological conditions at the cellular, organellar and molecular levels.


Quality


Each vial contains ≥ 1X106 viable cells.
Cells are tested negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
Cells are negative for mycoplasma contamination.
Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.


Storage and Stability


AC16 Human Cardiomyocyte Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Quality Level100
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable

There are no downloads for this product.