Anti-CD73; clone AA60-E3-3

Stock Code: 3572050
Manufacturer Part No: MABD122
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Analysis Note


ControlHuman Adipose Mesenchymal Stem Cells


Application


Detect CD73 using this mouse monoclonal antibody, Anti-CD73 Antibody, clone AA60-E3-3 validated for use in ICC, Flow Cytometry, western blotting & IP.


Research CategoryStem Cell Research


Research Sub CategoryMesenchymal Stem Cells


This antibody has been shown to work in immunocytochemical applications on human adipose mesenchymal stem cells. This antibody has been shown to work in western blot applications on HMEC-1 cells by an independant lab.This antibody has been shown to work in immunoprecipitation applications on HMEC-1 cells by an independant lab.


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


CD73, also known as Ecto-5′-nucleotidase, catalyzes the conversion of purine 5′ mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation, being expressed on subsets of B-cells and T-cells. CD73 is also a marker of undifferentiated mesenchymal stem cells and is often used to identified stem cell populations.


Immunogen


Whole Mouse Dermal Lymphatic Endothelial Cells (DLEC)


Linkage


Replaces: MABC668


Other Notes


Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


Physical form


Protein G Purified


Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.


Format: Purified


Quality


Evaluated by Flow Cytometry on human adipose mesenchymal stem cells.
Flow Cytometry Analysis: 1 µg/mL of this antibody detected CD73 on 1X10^6 human adipose mesenchymal stem cells.


Storage and Stability


Stable for 1 year at 2-8°C from date of receipt.


Target description


63 kDa calculated

Quality Level100
ManufacturerSIGMA-ALDRICH

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