Application
This Anti-Stage-Specific Embryonic Antigen-3 Antibody, clone MC-631 is validated for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence and Immunohistochemistry for the detection of Stage-Specific Embryonic Antigen-3.
Research CategoryStem Cell Research
Immunocytochemistry Analysis: 10µg/mL from a representative lot detected Stage-Specific Embryonic Antigen-3 in H9 cells.
Immunocytochemistry Analysis: A representative lot detected SSEA-3 immunoreactivity among T
cell-derived iPSCs (TiPSCs) by fluorescent immunocytochemistry (Kishino, Y., et al. (2014). PLoS One. 9(5):e97397).
Immunocytochemistry Analysis: A representative lot detected the presence of SSEA-3-positive cancer stem cells (CSCs) among cultured HCT116 colorectal cancer (CRC) cells by fluorescent immunocytochemistry (Suzuki, Y., et al. (2013). Int. J. Oncol. 42(1):161-167).
Immunocytochemistry Analysis: A representative lot detected SSEA-3 immunoreactivity among induced pluripotent stem (iPS) cells from human molars-derived mesenchymal stromal cells (MSCs) by fluorescent immunocytochemistry (Oda, Y., et al. (2010). J. Biol. Chem. 285(38):29270-29278).
Flow Cytometry Analysis: A representative lot detected the presence of SSEA-3-positive cancer stem cells (CSCs) in five cultured colorectal cancer (CRC) cell lines, HCT116, Caco-2, DLD-1, HT-29, and SW480 (Suzuki, Y., et al. (2013). Int. J. Oncol. 42(1):161-167).
Immunofluorescence Analysis: A representative lot detected a mall number of SSEA-3-positive stromal cells in normal colorectal epithelia and SSEA-3-positive cancers in colorectal adenocarcinomas by fluorescent immunohistochemistry using frozen tissue sections (Suzuki, Y., et al. (2013). Int. J. Oncol. 42(1):161-167).
Research Sub CategoryCell Cycle, DNA Replication & Repair
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Stage-specific embryonic antigen 3 (SSEA-3) is a glycosphingolipid composed of five carbohydrate units connected to a sphingolipid. Sphingolipids are key players in cell signaling and the SSEA-3 is a well known marker of stem cells (ESCs, MSCs, iPSCs, CSCs) differentiation, where SSEA-3 rapidly disappears when the cells start to differentiate.
Immunogen
4-8 cell stage mouse embryos.
Linkage
Replaces: MAB4303
Other Notes
Concentration: Please refer to lot specific datasheet.
Physical form
Purified by Ion-Exchange Chromatography
Format: Purified
Purified rat monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
Quality
Evaluated by Flow Cytometry in H9 cells.
Flow Cytometry Analysis: 1.0 µg of this antibody detected Stage-Specific Embryonic Antigen-3 in H9 cells.
Specificity
