Anti-Nestin; clone rat-401; Alexa Fluor

Anti-Nestin; clone rat-401; Alexa Fluor

Stock Code: 3572003
Manufacturer Part No: MAB353A4
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Analysis Note


ControlMouse cortical neural stem cells (SCR029)


Application


Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).


Detect the Nestin protein using this Anti-Nestin, clone rat-401, Alexa Fluor 488 conjugate validated for use in ICC.


Research Sub CategoryNeurofilament & Neuron MetabolismNeuronal & Glial Markers


Research CategoryStem Cell ResearchNeuroscience


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.


Immunogen


Embryonic rat spinal cord


Legal Information


ALEXA FLUOR is a registered trademark of Life Technologies


Other Notes


Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


Physical form


Protein G Purified


Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.


Quality


Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).


Specificity



This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.


Storage and Stability


Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.


Target description


The unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDa

ManufacturerSIGMA-ALDRICH

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