HAT Media Supplement (50x) Hybri-Max, gamma-irradiated, lyophilized powder, BioXtra, suitable for hy

HAT Media Supplement (50x) Hybri-Max, gamma-irradiated, lyophilized powder, BioXtra, suitable for hy

Stock Code: 3571845
Manufacturer Part No: H0262-10VL
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Application


HAT Media Supplement (50×) Hybri-Max™ has been used:in the selection and validation of hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient clonesin the generation of mAbs (monoclonal antibodies)in the luciferase-based bioassay (LUC) assay


Legal Information


Hybri-Max is a trademark of Sigma-Aldrich Co. LLC


Reconstitution


Reconstitute contents of vial with 10?ml sterile cell culture medium. Stock solution is sufficient to prepare 500?ml medium. Final working concentration: 100 µM hypoxanthine, 0.4 µM aminopterin, 16 µM thymidine.

Quality Level200
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - hybridoma: suitable
Product LineBioXtra
Storage Temp.−20°C
Impuritiesendotoxin, tested
Formlyophilized powder

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