HT Media supplement is a cocktail hypoxanthine and thymidine qualified for use in hybridoma selection and cell culture applications. HT Media Supplement (50×) Hybri-Max™ has been used as a supplement in INS-1 ?-cells co-culture medium. It is suitable for use as medium for hybridoma cells and as Dulbecco?s Modified Eagle Medium (DMEM) for HeLa S3 and MTH1-KO cells.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Reconstitution
Reconstitute contents of vial with 10?ml sterile cell culture medium. Stock solution is sufficient to prepare 500?ml medium. Final working concentration: 100 µM hypoxanthine, 16 µM thymidine.