FGF-acidicHumanRecombinantAnimalFree50ug

FGF-acidicHumanRecombinantAnimalFree50ug

Stock Code: 3571811
Manufacturer Part No: GF321
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Application


Research CategoryStem Cell Research


Research Sub CategoryGrowth Factors & Receptors


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGFacidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGFacidic is a 16.0 kDa protein consisting of 141 amino acid residues.


Product Source: Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.


Physical form


Product is presented in 5 mM Sodium Phosphate, pH 8.0 + 100mM NaCl. and is filtered through a 0.2 micron filter before lyophilization.


Quality


Bioactivity assay:
Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 10 ng/ml corresponding to a specific activity of ≥ 1 x 10^5 units/mg.
Assay #2: The ED50 was determined by a cell proliferation
assay using balb/c 3T3 cells is ≤ 0.5 ng/ml in the presence
of 10 µg/ml heparin, corresponding to a specific activity of
≥ 2 x 10^6 units/mg.


Storage and Stability


Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in 5 mM Sodium Phosphate, pH 8.0 to a concentration of ﹤0.5 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.

Quality Level100
ManufacturerSIGMA-ALDRICH
Impurities﹤0.1 µg/mg protein endotoxin (<1 EU/µg)

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