COLLAGEN V; HU; PUR-100UG

COLLAGEN V; HU; PUR-100UG

Stock Code: 3571522
Manufacturer Part No: CC077
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Application


Human Collagen Type V has been used to study the interaction of high endothelial venule protein (hevin) with human collagen V by fluorescence polarization (FP) assay and surface plasmon resonance(SPR). It has also been used to incubate the 96 well plate to determine the protein levels in media and cell lysates of fibroblasts.


Biochem/physiol Actions


Collagen type V acts as a regulator of collagen fibrillogenesis in the cornea and skin dermis. It interacts with matrix collagens and structural proteins thereby contributing to the structural integrity of tissues. Lower levels of type V collagen lead to loss of corneal transparency and Ehler Danlos syndrome.


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


Collagen type V is fibril-forming collagen that is located in the lung, bone, and fetal membranes along with type I collagen. It is minor collagen present in the extracellular matrix.


Legal Information


CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


Other Notes


Molecular composition: α1(V)]2 α2(V), native triple helix. Purity and retention of the native helical structure were monitored by SDS-PAGE, ORD measurement, and by reaction with anti-collagen type-specific monoclonal antibodies. Product Source: Human placenta, negative for HBsAg and HIV antibodies.


Physical form


Liquid, in 0.1M acetic acid, pH 3.0. No preservatives added.


Preparation Note


Purified by serial salt precipitations of a pepsin extraction of human fetal membranes and chromatography on DEAE-cellulose.


Storage and Stability


Maintain at -20°C in undiluted aliquots for up to 12 months. Do not thaw and refreeze.

ManufacturerSIGMA-ALDRICH
Impurities﹤1% collagen type III, ﹤2% collagen type IV, ﹤0.5% non-collagen proteins, ﹤2% collagen type I
Formliquid
Assay95% (SDS-PAGE)

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