E-Cadherin human, recombinant, expressed in E. coli, 0.5 mg protein/mL

E-Cadherin human, recombinant, expressed in E. coli, 0.5 mg protein/mL

Stock Code: 3571256
Manufacturer Part No: 5085-100UG
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Application


E-Cadherin has been used as coating matrix protein for 1) maintaining long-term ES or iPS cell culture then combine with proper ES cell culture media, 2) as a coating matrix material for 11R tag recombinant TF intracellular delivery for protein derived iPS protocol with extremely low-level non-specific interaction and 3) as a native antigen for antibody production.Use these recommendations as guidelines to determine the optimal coating conditions for your culture system. 1. Thaw E-Cadherin and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so that the volume added covers the surface evenly. Note: Use 1 ml PBS per well in a 6-well plate.2. Add 5 - 10 µg protein to each well and incubate at 2 to 10 °C overnight.3. After incubation, aspirate remaining material. 4. Plates are ready for use. They may also be stored at 2-8 °C damp or air dried if sterility is maintained.


Preparation Note


Recombinant human E-Cadherin gene (155-710 aa Fragment) was constructed with codon optimization and expressed in non-fusion protein form in E. coli as inclusion bodies. The final product was refolded using a unique "temperature shift inclusion body refolding" technology and chromatographically purified.


Sequence


MDWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIP

ManufacturerSIGMA-ALDRICH
Further Info0.1 mg recombinant human E-Cadherin in 20 mM Tris-HCl buffer, containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol.
Formliquid
Assay≥90% (SDS-PAGE)

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