apo-Transferrin bovine, >=97%
Application
All cells require iron for the proper uptake of oxygen from their environment. Transferrin, a protein isolated from serum, causes the uptake and transport of iron from culture medium to the cells. This allows proper oxygen uptake and also stimulates growth-related enzyme activity. Iron-saturated transferrin (holo transferrin) is continually recycled after releasing iron and becomes iron-deficient transferrin (apo-transferrin). Choosing which form to use depends upon the culture conditions. In media such as Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12 (DMEM/F12) that contain high levels of iron salt, using apo-transferrin is preferred.
Packaging
100, 500 mg in poly bottle
| Quality Level | 200 |
|---|---|
| Manufacturer | SIGMA-ALDRICH |
| Technique(s) | cell culture - mammalian: suitable |
| Storage Temp. | 2-8°C |
| Solubility | H2O: 20 mg/mL |
| Form | powder |
| Assay | ≥98% |
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