Fluorescein di(beta-D-galactopyranoside),
Application
Fluorescein di(?-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).
Fluorescein di(?-D-galactopyranoside) has been used as a substrate in ?-galactosidase assay.
Biochem/physiol Actions
Fluorescein di (?-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by ?-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using ?-galactosidase (Lac Z) as a reporter gene.
Packaging
1, 5, 25 mg in glass insert
Substrates
Fluorogenic substrate for β-galactosidase
| Quality Level | 100 |
|---|---|
| InChI key | ZTOBILYWTYHOJB-WBCGDKOGSA-N |
| InChI | 1S/C32H32O15/c33-11-21-23(35)25(37)27(39)30(45-21)42-13-5-7-17-19(9-13)44-20-10-14(43-31-28(40)26(38)24(36)22(12-34)46-31)6-8-18(20)32(17)16-4-2-1-3-15(16)29(4 |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | −20°C |
| Solubility | methanol: 20 mg/mL, clear, yellow |
| Form | powder |
| Assay | ≥98% (HPLC) |
There are no downloads for this product.
