G 418 disulfate salt, plant cell culture tested, BioReagent
Application
G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 µg/mL or less while animal cells may require 300-500 µg/mL.
Caution
The product is stable for three years when stored at 2-8°C.
General description
Chemical structure: aminoglycoside
Packaging
250 mg in glass bottle
1, 5 g in glass bottle
Preparation Note
G418 powder is soluble in water at 100 mg/mL.
| Colour | white to off-white |
|---|---|
| InChI key | UHEPSJJJMTWUCP-NKCAIAFTSA-N |
| InChI | 1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23 |
| Manufacturer | SIGMA-ALDRICH |
| Form | powder |
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