Photobiotin acetate salt, For labeling DNA probes for hybridizations
Application
Photobiotin acetate salt has been used to label DNA probes to perform DNA?DNA hybridization to determine genomic relatedness.
Biochem/physiol Actions
Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins. Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.
Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.
Caution
Light sensitive.
General description
Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.
| Quality Level | 200 |
|---|---|
| InChI key | FFBLNTOMOSLSQM-AYEYRVMASA-N |
| InChI | 1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22, |
| Manufacturer | SIGMA-ALDRICH |
| Solubility | H2O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.) |
| Form | film or powder |
| Assay | ≥95% |
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