Pyruvate Carboxylase from bovine liver, buffered aqueous glycerol solution, 5-25 units/mg protein (B
Application
Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes.
The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.
Biochem/physiol Actions
Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA.
Physical form
Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA.
Preparation Note
Affinity purified
Unit Definition
One unit will convert 1.0 µmole of pyruvate and CO2 to oxalacetate per min at pH 7.8 at 30?°C.
| Quality Level | 200 |
|---|---|
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | −20°C |
| Form | buffered aqueous glycerol solution |
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