Aldolase from rabbit muscle, lyophilized powder, >=8.0 units/mg protein
Application
Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 . Product A2714 is essentially sulfate-free and contains citrate buffer salts.
Biochem/physiol Actions
Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the ?/? 8 barrel fold .
Packaging
100, 200, 500 units in poly bottle
Physical form
Essentially sulfate-free containing citrate buffer salts
Unit Definition
One unit will convert 1.0?µmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH?7.4 at 25?°C.
| Quality Level | 300 |
|---|---|
| Manufacturer | SIGMA-ALDRICH |
| Composition | Protein, ≥80% biuret |
| Storage Temp. | −20°C |
| Form | lyophilized powder |
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