3, 3,5 ,5-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA, ready to use solution
Application
3, 3?,5 ,5?-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase:to determine synthesized hyaluronan and HAS2 (hyaluronan-synthesizing enzyme) expression by the transfectantto measure the levels of ?-hemolysin toxinin imaging bound IgGs
3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.
General description
This product contains 3, 3′,5 ,5′-Tetramethylbenzidine (TMB) in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.
| Quality Level | 200 |
|---|---|
| InChI key | UAIUNKRWKOVEES-UHFFFAOYSA-N |
| InChI | 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3 |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | 2-8°C |
| Form | aqueous solution |
There are no downloads for this product.
