3,3,5,5-Tetramethylbenzidine, >=98% (TLC)
Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
3,3?,5,5?-Tetramethylbenzidine has been used:as a supplement in Man-Rodosa-Sharpe (MRS) agar plate, to stainsthe lactobacillicolonies blue in the presence of O2as a substrate solution for ELISA (enzyme-linked immunosorbent assay)in myeloperoxidase activity assay to quantify neutrophil
Biochem/physiol Actions
3,3?,5,5?-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in insitu free available chlorine (FAC) monitoring of chlorite-based sanitizers.
Packaging
1, 5, 100 g in glass bottle
Bottomless glass bottle. Contents are inside inserted fused cone.
100 mg in glass bottle
| Quality Level | 200 |
|---|---|
| mp | 168-171 °C (lit.) |
| InChI key | UAIUNKRWKOVEES-UHFFFAOYSA-N |
| InChI | 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3 |
| Manufacturer | SIGMA-ALDRICH |
| Solubility | ethyl acetate: 50 mg/mL, clear to very slightly hazy |
| Form | powder |
| Assay | ≥98% (TLC) |
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