o-Phenylenediamine dihydrochloride, tablet, 10 mg substrate per tablet
Application
o-Phenylenediamine dihydrochloride has been used as a substrate for horseradish-peroxidase-conjugated secondary antibody in enzyme-linked immunosorbent assay (ELISA).
General description
o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.
o-Phenylenediamine (o-PD) is a colorless and nonfluorescent molecule.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 µl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
| Quality Level | 300 |
|---|---|
| mp | 258 °C (dec.) (lit.) |
| InChI key | RIIWUGSYXOBDMC-UHFFFAOYSA-N |
| InChI | 1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | 2-8°C |
| Form | tablet |
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