4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate
Application
4-Nitrophenyl phosphate di(tris) salt has been used:as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysatesto determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.
| Quality Level | 100 |
|---|---|
| InChI key | XXAXKCWOTRABOW-UHFFFAOYSA-N |
| InChI | 1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2 |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | −20°C |
| Solubility | water: 50 mg/mL, clear, colorless to light yellow |
| Form | powder |
| Assay | ≥97% (enzymatic) |
There are no downloads for this product.
