Naphthol AS-BI phosphate disodium salt hydrate, >=99.0% (HPLC)
Application
Naphthol AS-BI phosphate disodium salt hydrate is suitable to use:in tartrate-resistant acid phosphatase (TRAP) stainingto detect osteoclast activityas a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activityto perform alkaline phosphatase reaction
Biochem/physiol Actions
Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.
General description
Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) is a substituted naphthol.
Substrates
Substrate for the histochemical demonstration of acid and alkaline phosphatase.
| Quality Level | 200 |
|---|---|
| InChI key | WNPKMQCBZKBNOS-UHFFFAOYSA-L |
| InChI | 1S/C18H15BrNO6P.2Na.H2O/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24;;;/h2-10H,1H3,(H,20,21)(H2,22,23,24);;;1H2/q;2*+1;/p- |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | −20°C |
| Solubility | water: 50 mg/mL, clear, colorless to yellow |
| Form | powder |
| Assay | ≥96.0% (HPLC) |
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