4-Methylumbelliferyl phosphate, phosphatase substrate

4-Methylumbelliferyl phosphate, phosphatase substrate

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Application


4-Methylumbelliferyl phosphate has been used as a substrate for alkaline phosphatase in the peptide-binding assay. It has also been used as a fluorogenic substrate in enzyme-linked immunosorbent assay (ELISA).


Biochem/physiol Actions


4-Methylumbelliferyl phosphate serves as a fluorogenic substrate for calmodulin-dependent protein phosphatase and in kinetic studies of alkaline phosphatase. It is a substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. 4-Methylumbelliferyl phosphate is seven times more sensitive than phenolphthalein monophosphate and 8-13 times more sensitive than with p-nitrophenyl phosphate when used in enzyme immunoassays for the detection of antibodies to human immunodeficiency viruses.


Packaging


1, 5 g in poly bottle


100, 250 mg in poly bottle


Substrates


Fluorogenic substrate for phosphatases.

Quality Level200
InChI keyBCHIXGBGRHLSBE-UHFFFAOYSA-N
InChI1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
ManufacturerSIGMA-ALDRICH
Further Infophosphatase substrate
Formpowder
Assay≥98% (HPLC)

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