4-Methylumbelliferyl sulfate potassium salt, sulfatase substrate

Descriptions

Application

4-Methylumbelliferyl sulfate potassium salt has been used as a substrate in coupled fluorescence sulfotransferase assay to regenerate 3?-phosphoadenosine 5?-phosphosulfate (PAPS) from 3?-phosphoadenosine 5?-phosphate for cerebroside sulfotransferase (CST) enzyme activity measurements. It has also been used as a substrate in arylsulfatase assay/4-methylumbelliferyl sulfate (4-MUS) assay to test the enzymatic activity of the protein-bead complex.

Biochem/physiol Actions

4-Methylumbelliferyl sulfate (4MUS) is capable of undergoing desulfation and involve in futile cycling with 4MU. It can be utilized to measure the arylsulfatase activity of several sulfatases.

General description

4-Methylumbelliferyl sulfate (4MUS), a general substrate, is a polar metabolite of 4-methylumbelliferone (4MU).

Packaging

1 g in poly bottle

500 mg in glass bottle

Specifications

Quality Level	200
InChI key	CSOCSPXOODWGLJ-UHFFFAOYSA-M
InChI	1S/C10H8O6S.K/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14;/h2-5H,1H3,(H,12,13,14);/q;+1/p-1
Manufacturer	SIGMA-ALDRICH
Solubility	water: 5 mg/mL, clear, colorless
Form	powder
Assay	≥99% (HPLC)