4-Methylumbelliferyl beta-D-cellobioside, glucanase substrate
Application
4-Methylumbelliferyl ?-D-cellobioside has been used as a substrate: to assay cellulase fromA. nigerto determine the extracellular enzyme activity for 1,4-?-cellobiohydrolaseto assay the activity of bound enzyme, endoglucanase I (EGIc) Trichoderma reeseiclass II hydrophobins (HFBI)
Biochem/physiol Actions
4-Methylumbelliferyl β-D-cellobioside is used as a substrate for cellulase assays. It is mainly used for activity staining of cellulases in polyacrylamide gels.
Packaging
100, 500 mg in glass bottle
| Quality Level | 200 |
|---|---|
| InChI key | PRTGXBPFDYMIJH-MKQZUAMYSA-N |
| InChI | 1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12- |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | 2-8°C |
| Solubility | water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow |
| Form | powder |
| Assay | ≥98.00% (TLC) |
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