1-Fluoro-2,4-dinitrobenzene, >=99%
Application
1-Fluoro-2,4-dinitrobenzene has been used to induce severe contact hypersensitivity in mice and to monitor gene expression by in vivo imaging method, and to induce chronic itch as a model for analysing itch-scratching behavior.
Biochem/physiol Actions
1-Fluoro-2,4-dinitrobenzene is a contact-sensitizing hapten, commonly used in experimental studies on contact hypersensitivity, inducing itch model, and atopic dermatitis. Mast cell activation is a key step in the DNFB induced sensitivity. DNFB covalently binds to the N-terminal amino acid of the protein and aids in Sanger sequencing.
General description
1-Fluoro-2,4-dinitrobenzene (DNFB) is a reagent that modifies the N-terminal amino acids of proteins and peptides. DNFB is also commonly known as Sanger′s Reagent, named after Frederick Sanger, who reported in a classic 1945 paper the use of DNFB for derivatization of proteins and peptides specifically at their N-termini.†
Packaging
10, 25, 100 mL in glass bottle
| Quality Level | 200 |
|---|---|
| mp | 25-27 °C (lit.) |
| InChI key | LOTKRQAVGJMPNV-UHFFFAOYSA-N |
| InChI | 1S/C6H3FN2O4/c7-5-2-1-4(8(10)11)3-6(5)9(12)13/h1-3H |
| bp | 178 °C/25 mmHg (lit.) |
| Manufacturer | SIGMA-ALDRICH |
| Density | 1.482 g/mL at 25 °C (lit.) |
| Form | solid |
| Assay | ≥99% |
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