5-Bromo-4-chloro-3-indolyl beta-D-galactopyranoside, >=98%

Descriptions

Application

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

5-Bromo-4-chloro-3-indolyl ?-D-galactopyranoside has been used to confirm transduction of Caki-2/LacZ clones. It has also been used in blue-white selection of transformants.

Packaging

10, 100, 500 mg in poly bottle

Reconstitution

A stock solution (100?mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20?°C and protected from light. Sterilization is not required.

Specifications

Quality Level	200
InChI key	OPIFSICVWOWJMJ-AEOCFKNESA-N
InChI	1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
Manufacturer	SIGMA-ALDRICH
Storage Temp.	−20°C
Solubility	DMF: methanol (1:1): soluble 50 mg/mL
Form	powder
Assay	≥98%