5-Bromo-4-chloro-3-indolyl phosphate disodium salt, >=98% (HPLC)
Application
5-Bromo-4-chloro-3-indolyl phosphate disodium salt has been used to detect protein-antibody complexes. It has also been used for tissue staining in alkaline phosphatase histochemistry.
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Legal Information
BCIP is a registered trademark of Sigma-Aldrich Co. LLC
Packaging
1, 5 g in poly bottle
500 mg in poly tube
25, 50, 100 mg in poly bottle
Substrates
Histochemical substrate for alkaline phosphatase.
| Quality Level | 200 |
|---|---|
| InChI key | OAZUOCJOEUNDEK-UHFFFAOYSA-L |
| InChI | 1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2 |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | −20°C |
| Solubility | DMF: insoluble, H2O: 20 mg/mL |
| Form | powder |
| Assay | ≥98% (HPLC) |
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