L-Alanine 4-nitroanilide hydrochloride, protease substrate
Application
L-Alanine 4-nitroanilide hydrochloride has been used to determine the activity of aminopeptidase in the intestinal segments and small intestine (jejunum and ileum).
Biochem/physiol Actions
L-Alanine-4-nitroanilide is used as a substrate to determine alanine aminopeptidase (AAP) activity. It is mainly chosen as a substrate, due to its relative specificity, higher hydrolysis rate and better solubility.
| Quality Level | 200 |
|---|---|
| InChI key | YEXRLSXNWLNHQR-RGMNGODLSA-N |
| InChI | 1S/C9H11N3O3.ClH/c1-6(10)9(13)11-7-2-4-8(5-3-7)12(14)15;/h2-6H,10H2,1H3,(H,11,13);1H/t6-;/m0./s1 |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | −20°C |
| Solubility | water: 50 mg/mL, clear to very slightly hazy |
| Form | powder |
| Assay | ≥99% (TLC) |
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