4-Aminophthalhydrazide,
Application
4-Aminophthalhydrazide has been used in:chemiluminescence-based reactive oxygen species (ROS) assay in bone marrow (BM) neutrophils post-Yersinia pseudotuberculosis infectionneutrophil ROS assays post-K. pneumonia exposure neutrophil ROS assays in response to formylated-methionine-leucine-proline
Biochem/physiol Actions
4-Aminophthalhydrazide or isoluminol is a chemiluminescent compound that comprises a phthalazine ring. It is more hydrophilic than luminol. Isoluminol is commercially used in a wide variety of in vitro immunoassays and is a chemiluminescence amplifier for studying reactive oxygen species. It is also useful as an electroluminescence (ECL) biosensor and as a probe for rapid microbial detection.
Features and Benefits
Produces chemiluminescence.
Packaging
1, 5, 10 g in glass bottle
| Quality Level | 200 |
|---|---|
| mp | 300 °C (lit.) |
| InChI key | HUDPLKWXRLNSPC-UHFFFAOYSA-N |
| InChI | 1S/C8H7N3O2/c9-4-1-2-5-6(3-4)8(13)11-10-7(5)12/h1-3H,9H2,(H,10,12)(H,11,13) |
| Manufacturer | SIGMA-ALDRICH |
| Solubility | 2 M NH4OH: 50 mg/mL, clear to slightly hazy |
| Form | powder |
| Assay | ≥98% (HPLC) |
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