SSC Buffer, powder blend
Application
Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for: Preparing nucleic acids for hybridization Hybridization buffer for Northern and Southern blots Washing buffer for Northern and Southern blots
Components
Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.
Features and Benefits
Multi-purpose use in hybridizations Easily reconstituted and diluted to any concentration needed
General description
SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.
| Quality Level | 200 |
|---|---|
| pH | 6.8-7.2 (20-25 °C, 263 mg/L in water) |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | room temp |
| Solubility | water: 263 mg/mL, clear, colorless |
| Form | powder blend |
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