DL-Dithiothreitol, for electrophoresis, >=99%
Analysis Note
Tested for use in reduced SDS-PAGE
Application
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Packaging
1, 5, 25 g in poly bottle
| Quality Level | 200 |
|---|---|
| Pack | 25G |
| mp | 40-44 °C, 41-44 °C (lit.) |
| InChI key | VHJLVAABSRFDPM-QWWZWVQMSA-N |
| InChI | 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1 |
| Manufacturer | SIGMA-ALDRICH |
| Form | powder |
| Assay | ≥99% |
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