phiX174 DNA Marker Hae III Digest, for DNA electrophoresis
Components
Sigma’;s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
General description
Sigma’;s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Other Notes
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Preparation Note
φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.
| Quality Level | 200, 100 |
|---|---|
| Pack | 20UG |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | −20°C |
| Suitability | suitable for electrophoresis (DNA) |
| Form | liquid |
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