X-GlcA, reagent for selection of recombinant bacterial clones
Application
Chromogenic substrate for β-glucuronidase (GUS) gene detection.
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.X-GlcA has been used in histochemical staining of root sections for light microscopic observation.
General description
X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Principle
When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.
| Quality Level | 200 |
|---|---|
| InChI key | JXCKZXHCJOVIAV-CYRSAHDMSA-N |
| InChI | 1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10- |
| Manufacturer | SIGMA-ALDRICH |
| Solubility | DMF: soluble |
| Form | powder |
| Assay | ≥98% (TLC) |
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