MPS1-IN-3

Descriptions

Application

Mps1-IN-3 has been used in 2D-proliferation and 3D-organotypic culture outgrowth assays.

Biochem/physiol Actions

Mps1 mediates phosphorylation of kinetochore scaffold 1.

Mps1-IN-3 is an inhibitor of Monopolar spindle 1 (MPS1, also known as TTK), a Dual-specificity kinase required for spindle pole body duplication and spindle checkpoint function. Mps1-IN-3 inhibited Mps1 kinase activity with an IC₅₀ value of 50 nM inhibited the proliferation of U251 glioblastoma cells as determined by cell counting with an IC₅₀ value of approximately 5 µM, and sensitized glioblastoma cells to vincristine in orthotopic mouse models.

General description

Monopolar spindle 1 (Mps1) protein contains a TPR (tetratricopeptide repeat) motif at its N-terminal and a C-terminal kinase domain.

Packaging

5, 25 mg in glass bottle

Specifications

Colour	light yellow to dark yellow
Quality Level	100
InChI key	OFRMASLPWOMYHN-UHFFFAOYSA-N
InChI	1S/C26H31N7O4S/c1-16(2)38(35,36)22-7-5-4-6-20(22)29-25-23-24(28-15-27-23)31-26(32-25)30-19-9-8-17(14-21(19)37-3)33-12-10-18(34)11-13-33/h4-9,14-16,18,34H,10-13
Manufacturer	SIGMA-ALDRICH
Solubility	DMSO: 20 mg/mL, clear
Form	powder
Assay	≥98% (HPLC)