Trypan Blue, powder, BioReagent, suitable for cell culture
Application
Trypan blue has been used:in cell viability assayto determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.to stain plant tissue in order to to visualize cell death
Biochem/physiol Actions
The trypan blue dye is useful to differentiate the dead and live cells based on the cell?s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.
General description
The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.
Packaging
5, 25, 100 g in glass bottle
| Pack | 25G |
|---|---|
| mp | >300 °C (lit.) |
| InChI key | GLNADSQYFUSGOU-UHFFFAOYSA-J |
| InChI | 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12 |
| Manufacturer | SIGMA-ALDRICH |
| Composition | Dye content, ~40% |
| Form | powder |
There are no downloads for this product.
