TRANSCRIPTOR HIFI CDNA SYNTH. KIT 200 RX

Descriptions

Application

The Transcriptor High Fidelity cDNA Synthesis Kit is designed to reverse transcribe RNA with increased fidelity compared to other reverse transcriptases. The kit features Transcriptor High Fidelity Reverse Transcriptase, a blend of a recombinant reverse transcriptase and a proofreading mediating enzyme optimized for two-step RT-PCR, making this the product of choice for:Cloning genes of interestSequencing transcriptomesGenerating cDNA libraries with large and full-length insertsGenerating gene expression analysis, like RNA splicing analysisAs the kit is also tested with the LightCycler^® Instruments and other real-time PCR instruments, the product is also ideal for quantitative RT-PCR applications that require high fidelity.The kit contains all components required for synthesizing cDNA suitable for direct use in qualitative RT-PCR with conventional thermal cyclers or quantitative RT-PCR on real-time PCR instruments. The 50-reaction pack size also includes 10 control reactions (control RNA and control primer mix).

Components

Transcriptor High Fidelity cDNA Synthesis Kit includes the following: Transcriptor High Fidelity Reverse Transcriptase (blend of a recombinant reverse transcriptase and a proofreading mediating enzyme) Transcriptor High Fidelity Reaction Buffer, 5x concentrated Protector RNase Inhibitor dNTP mix, PCR Grade Anchored-oligo (dT)₁₈Primer Random Hexamer Primer DTT Water, PCR Grade Control RNA – total RNA fraction purified from the immortalized cell line K-562* Control PBGD primer mix (forward and reverse primer)** Included only in the 50-reaction pack size.

Features and Benefits

Increase accuracy during reverse transcription reactions. Benefit from an optimized enzyme blend with 7-fold higher fidelity compared to other commonly used reverse transcriptases. Rely on true-fidelity data, as determined by the sequencing of several million bases with the Genome Sequencer 20 System.Combine accuracy with high sensitivity, high yield, and full-length transcripts. Generate full-length transcripts up to 14 kb with the anchored-oligo (dT)₁₈ primers included in the kit . Obtain excellent yields. Detect low-copy number templates; reverse transcribe from as little as 10 pg template RNA. Transcribe a variety of templates, even the most difficult ones (e.g., GC-rich RNAs with high secondary structure) at temperatures up to +55°C.Get results faster. Reduce reverse transcription reaction time to as little as 10 minutes. Be first to finish

General description

Retroviral reverse transcriptases commonly used for cDNA synthesis exhibit a higher error rate than other DNA polymerases used in nucleic acid analysis techniques. This lack in accuracy leads to a significant number of base exchanges or frameshifts, which are further propagated in subsequent PCR reactions. High fidelity (proofreading) PCR enzymes have been available for many years; Roche’;s new high accuracy reverse transcriptase is further able to synthesize high yields of full-length cDNA.High mutation rate is a hallmark of retrovirus replication. This originates in the mechanism of genome replication by the viral-encoded reverse transcriptase, which converts the genomic RNA of the virus to a dsDNA