500mL McCoys 5A (Iwakata and Grace Modification) with L-glutamine
McCoy's 5A medium was originally formulated to meet the culture requirements of Novikoff Hepatoma cells, and has been found to support the indefinite proliferation of Walker 256 carcinoma cells along with a wide range of human and rat normal and transformed cells. Originally modified by Iwakata and Grace, this version of McCoy's 5A includes increased levels of inositol and glucose.
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