Oz Biosciences F-Luc Reporter Gene mRNA
F-Luc mRNA has been designed to produce high expression level of FireFly luciferase. It is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin. OZ Biosciences mRNAs are produced by in vitro transcription and stabilised at the 5' end by modified nucleotides capping (Cap1). These mature mRNAs contain a poly(A) tail at the 3' end and are also optimized to yield improved stability and performance.
Ready-to-use stabilised F-Luc mRNA
Concentration: 1.0mg/mL in 1mM sodium citrate, pH 6.4
MRNA16 does not bear any additional nucleotide modifications
MRNA12 is modified with 5-methoxyuridine (5moU) to reduce innate immune response
MRNA24 is modified with N1-methyl-pseudouridine (N1-mψ) to reduce innate immune response
MRNA12c and MRNA16c are labelled with Cy5 (Sulfo-Cyanine5) by replacing 15% of U or moU by UTP-Cy5
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